From the 764 samples that were selected for phase one metagenomic wgs sequencing, a subset of 12 stool samples underwent supplementary sequencing using the 454 FLX Titanium platform, and were assembled using Newbler (454/Roche GS Mapper/Assembler). Assemblies underwent gene prediction and annotation to generate the HMP Hybrid Gene Index.
Gene sequence and annotation is provided here in gff3 format, with one file per sample. Functional attributes assigned to each gene prediction include gene name, gene symbol, Gene Ontology (GO) assignments and Enzyme Commission (EC) numbers.
Protocols and Tools
- QC & Sample Review - coming soon
- Gene Prediction - coming soon
- Metagenomics Annotation SOP