Structural annotation of co-assembled contigs was performed using Metagenemark-3.25. Translations of the resulting gene predictions were used as input for searches against Uniref100, Pfam and TIGRfam2 HMM models, TMHMM for identification of transmembrane helices, and lipoprotein motifs. Annotation was assigned using a hierarchical scheme of evidence input, providing common names, gene symbols, EC numbers and GO terms, as applicable based on the highest quality evidence available. Annotation output is a gff3 file for each co-assembly.

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